Determination of salidroside and crenulatin in Rhodiolae Sacrae Herbra by HPLC-ELSD
10.7501/j.issn.0253-2670.2014.08.021
- Author:
Xiao-Yan WU
1
Author Information
1. Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Crenulatin;
HPLC-ELSD;
Quality standard;
Rhodiolae Sacrae Herbra;
Salidroside
- From:
Chinese Traditional and Herbal Drugs
2014;45(8):1157-1159
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a determination method for the contents of salidroside and crenulatin in Rhodiolae Sacrae Herbra, and to compare the content differences of the two compounds in Rhodiolae Sacrae Herbra from different batches. Methods: Using HPLC-ELSD method and Kromasil C18 column (250 mm × 4.6 mm, 5 μm), with acetonitrile and water as mobile phase, gradient elution at flow rate of 1.0 mL/min, column temperature of 30°C, nitrogen flow rate of 2.0 L/min, drift tube temperature of 45°C, and gain of 2. Results: The linear of salidroside was 0.806-8.06 μg (r = 0.9996, n = 6), and the average recovery rate was 100.56%. The linear of crenulatin was 2.142-21.42 μg (r = 0.9999, n = 6), and the average recovery rate was 100.40%. Conclusion: This method is simple, and accurate, and has better reproducibility for the determination of the two compounds in Rhodiolae Sacrae Herbra. It could be used as a quantitative determination method for salidroside and crenulatin in Rhodiolae Sacrae Herbra.
