Association of RNase3 Polymorphisms with the Risk of Colorectal Cancer.
10.3393/jksc.2010.26.1.45
- Author:
Yong KIM
1
;
Dong Baek KANG
;
Jeong Kyun LEE
;
Young Hwan LEE
;
In Hong KANG
;
Soo Cheon CHAE
;
Yun Sik YANG
;
Won Cheol PARK
Author Information
1. Department of Surgery, Wonkwang University College of Medicine, Iksan, Korea. parkwc@wonkwang.ac.kr
- Publication Type:Original Article
- Keywords:
RNase3;
Polymorphism;
Colorectal cancer
- MeSH:
Asthma;
Case-Control Studies;
Colonic Neoplasms;
Colorectal Neoplasms;
Eosinophils;
Freezing;
Gene Frequency;
Genotype;
Haplotypes;
Immune System;
Leukocytes;
Polymorphism, Single Nucleotide;
Rectal Neoplasms;
Ribonucleases
- From:Journal of the Korean Society of Coloproctology
2010;26(1):45-52
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: RNase3 is a secretory ribonuclease found in eosinophilic leukocytes and is involved in the innate immune system. Its cytotoxic activity is effective against a wide range of pathogens. Generally, high levels of RNase3 have been reported in cases of active asthma and allergic diseases. However, a relationship between RNase3 and colon cancer has not yet been reported. We performed a case-control study to examine the relationship between RNase3 polymorphisms and the risk of colorectal cancer in Korean people. METHODS: Blood sampling of each group was performed, TaqMan in g.-550A>G, PCR-RFLP in g.371C>G, and high resolution melting (HRM) in g.499C>G were analyzed. As results, the three SNPs, g.-550A>G, g.371C>G, and g.499C>G, in RNase3 and their haplotypes were analyzed. RESULTS: The genotype and the allele frequencies of RNase3 g.-550A>G and g.371C>G were not significantly associated with increased risk for colon cancer compared to the control group, but the RNase3 g.499C>C genotype was associated with a significantly increased risk for colorectal cancer compared to the control group (P=0.001). Also, the RNase3 g.499C>C genotype was more specifically associated with a significantly increased risk for right colon cancer than left colon cancer (P<0.001). In haplotypes of RNase3 SNPs, g.-550G, g.371C, and g.499G were significantly associated with colorectal cancer (P=0.019): more specifically, left colon cancer and rectal cancer than right colon cancer (P=0.048). CONCLUSION: The RNase3 g.499C>G polymorphism may have an influence on colorectal cancers and may have a more specific influence on right colon cancer than left colon cancer and on rectal cancer. However, the significance of the RNase3 g.-550A>G and g.371C>G polymorphisms need careful interpretation and require confirmation in larger studies.