Cloning and identification of two squalene synthase genes from Salvia miltiorrhiza
10.7501/j.issn.0253-2670.2014.09.021
- Author:
Yi-Mian MA
1
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College
- Publication Type:Journal Article
- Keywords:
Gene cloning;
Gene expression;
Salvia miltiorrhiza Bunge;
Squalene synthase;
Tritepenoids
- From:
Chinese Traditional and Herbal Drugs
2014;45(9):1307-1312
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To clone and identify the squalene synthase genes in Salvia miltiorrhiza. Methods: The primers were designed based on the predication result of S. miltiorrhiza genome genes and two squalene synthase genes (SmSQS1 and SmSQS2) from S. miltiorrhiza were amplified via RT-PCR method. Some bioinformatic methods and softwares were used for the gene structure analysis, the analyses of sequence homology and protein conserved domains of the two gene encoding polypeptides were carried. Real-time quantitative PCR (RT-qPCR) method was used for the analysis of gene expression patterns. Results: The two squalene synthase genes of S. miltiorrhiza were obtained by RT-PCR method. The encoded polypeptide of the two genes has the related domains and a conserved motif for squalene synthases. The two genes had the different exon/intron characteristics and the different tissue-specific and time-specific expression patterns. Conclusion: There are two squalene synthase genes existing in the genome of S. miltiorrhiza, which have the different gene structures and expression patterns, and probably play the different roles in the biosynthesis process of steroids and triterpenoids in S. miltiorrhiza.
