Stimultaneous determination of five flavonoids in Callicarpa nudiflora by UPLC
10.7501/j.issn.0253-2670.2014.10.022
- Author:
Jun SHAO
1
Author Information
1. School of Pharmacy, Nanchang University
- Publication Type:Journal Article
- Keywords:
5, 4'-dihydroxy-3, 7, 3'-trimethoxyflavone;
Acteoside;
Callicarpa nudiflora Hook.;
Luteolin;
Luteolin-4'-O-β-D-glucopyranoside;
Luteoloside;
UPLC
- From:
Chinese Traditional and Herbal Drugs
2014;45(10):1473-1476
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop a UPLC method for the stimultaneous determination of five active ingredients in Callicarpa nudiflora. Methods Analysis was performed on an Agilent Eclipse XDB-C18 column (100 mm ×3.0 mm, 1.8 μm) eluted with acetonitrile (A) and 0.1% methanoic acid (B) in a gradient program. The flow rate was 0.7 mL/min, the detection wavelength was 350 nm, and the column temperature was 40°C. Results Luteoloside, acteoside, luteolin-4'-O-β-D-glucopyranoside, luteolin, and 5, 4'-dihydroxy-3, 7, 3'-trimethoxyflavone showed a good linearity in the ranges of 2.44-122.0 μg/mL (r = 0.999 8), 9.06-453.0 μg/mL (r = 0.999 9), 4.42-221.0 μg/mL (r = 0.999 9), 3.36-168.0 μg/mL (r = 0.999 8), and 2.52-126.0 μg/mL (r = 0.999 8). The average recoveries, measured at three concentration levels, varied from 98.5%-100.8%. Conclusion The method is simple, accurate, and can be used for the quality control of C. nudiflora.
