Comparison on content differences of four bioactive compounds in different Wujiwan decoction by HPCE internal standard method
10.7501/j.issn.0253-2670.2014.11.014
- Author:
Feng-Yun JIN
1
Author Information
1. Guiyang College of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Berberine hydrochloride;
Capillary electrophoresis;
Compound granule;
Granule prescription;
Internal standard method;
Jatrorrhizine hydrochloride;
Paeoniflorin;
Palmatine hydrochloride;
Traditional decoction;
Wujiwan
- From:
Chinese Traditional and Herbal Drugs
2014;45(11):1573-1576
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the content difference of berberine hydrochloride, palmatine hydrochloride, jatrorrhizine hydrochloride, and paeoniflorin in Wujiwan traditional decoction, granule prescription, and compound granule using HPCE internal standard method. Methods: Capillary zone electrophoresis (CZE) method was used for a complete separation of the components, which was achieved with 50 mmol/L borax buffer-methanol (2:1), constant voltage of 25 kV, pressure injection 5 kPa × 15 s, electrolyle sealing 5 kPa × 10 s, and column temperature of 15°C. Results: The capillary electrophoresis method is simple and accurate. The contents of the three alkaloids in the granule prescription were obviously higher than those in traditional decoction and compound granule. Conclusion: This method is instructive for clinical application and quality control of Wujiwan prescription and compound granule.
