Establishment of rapid propagation system of Magnolia officinalis subsp. biloba
10.7501/j.issn.0253-2670.2014.12.023
- Author:
Ying-Zi MA
1
Author Information
1. College of Life Science and Technology, Central South University of Forestry and Technology
- Publication Type:Journal Article
- Keywords:
Magnolia officinalis subsp. biloba (Rehd.et Wils.) Law.;
Medium;
Orthogonal test;
Rapid propagation;
Tissue culture
- From:
Chinese Traditional and Herbal Drugs
2014;45(12):1769-1774
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a rapid propagation system of Magnolia officinalis subsp. biloba. Methods: Using peeling seeds of M. officinalis subsp. biloba as the initial explants, different media and various combinations of plant growth regulators (6-BA, NAA, IBA, and IAA) on the seeds germination, the seedlings subculture, and rooting culture were studied by single factor test and orthogonal test. Results: The best culture medium of seeds germination was B5 + 6-BA 0.5 mg/L + NAA 0.1 mg/L, and the budding percentage was 87.0%; The effective medium for cluster buds-inducing and subculture was MS + 6-BA 2.5 mg/L + NAA 0.5 mg/L, and the propagation coefficient was 6.2; The best rooting medium was 1/2 MS + NAA 0.5 mg/L, and the rooting rate was over 84% after 30 d. Conclusion: Rapid propagation technique system is established in order to lay technique foundation for the industrial production of M. officinalis subsp. biloba.
