Effects of oxymatrine on proliferation of hepatic stellate cells HSC-T6 and expression of telomerase
10.7501/j.issn.0253-2670.2014.14.018
- Author:
Yu-Lu ZHOU
1
Author Information
1. The Third Xiangya Hospital, Central South University
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Hepatic stellate cells;
HSC-T6;
Oxymatrine;
Telomerase;
Telomerase reverse transcriptase
- From:
Chinese Traditional and Herbal Drugs
2014;45(14):2051-2055
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the induction effect of oxymatrine on the apoptosis process in rat hepatic stellate cell line HSC-T6 and to define its impact on telomerase activity and mRNA expression of subunit telomerase reverse transcriptase (rTERT). Methods: HSC-T6 cells were cultivated with different concentration of oxymatrine for different time periods. Effect of oxymatrine on the growth inhibition of HSC-T6 cells was analyzed by MTT assay. Apoptosis of HSC-T6 cells was detected by flow cytometry. Telomerase activity was determined by TRAP-PAGE-silver staining and the expression of rTERT mRNA was examined by RT-PCR assay. Results: Oxymatrine significantly suppressed the growth of HSC-T6 cells and induced apoptosis, and also reduced the activity of telomerase and inhibited the rTERT-mRNA expression in HSC-T6 cells. Conclusion: The function that oxymatrine inhibits the proliferation of HSC-T6 cells may be associated with its action on the cellular telomerase and telomerase rTERT-mRNA activity.
