Optimization of diabetic gastroparesis rat model by orthogonal design
10.7501/j.issn.0253-2670.2014.14.017
- Author:
Guo-Tai WU
1
Author Information
1. Department of Pharmacology, Gansu College of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Alloxan;
Diabetic gastroparesis;
Model;
Motilin;
Orthogonal test
- From:
Chinese Traditional and Herbal Drugs
2014;45(14):2047-2050
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To optimize the preparation methods of diabetic gastroparesis rat model by orthogonal test. Methods: The diabetes gastroparesis rat model was duplicated with alloxan (ALX) by using four factors and three levels of orthogonal design. The fasting blood glucose, gastric emptying rate, and plasma motilin level were scored as evaluation indexes, and the influence of different factors on diabetic gastroparesis model rats was observed. Results: The effect of feed type on the diabetic gastroparesis rat model was not obvious, but the effects of modeling time and modeling agent ALX dose on diabetic gastroparesis rat model were decisive. The order of the importance was as follows: modeling time > dose of ALX > feed types. Conclusion: The optimized test method can effectively improve the diabetic gastroparesis rat model and provide a technical platform for the study on drug evaluation of diabetic gastroparesis.
