Simultaneous determination of sixteen ginsenosides in Panax ginseng and its preparation by HPLC
10.7501/j.issn.0253-2670.2014.14.009
- Author:
Chong GUO
1
Author Information
1. College of Traditional Chinese Medicine, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Compound K;
Ginsenoside;
Ginsenoside Rc;
Ginsenoside Rd;
Ginsenoside Re;
Ginsenoside Rf;
HPLC;
Panax ginseng C. A. Mey.;
Protopanaxadiol;
Protopanaxatriol
- From:
Chinese Traditional and Herbal Drugs
2014;45(14):2009-2013
- CountryChina
- Language:Chinese
-
Abstract:
Objective: In order to evaluate the quality of Panax ginseng and its preparation, a simple and accurate HPLC method for determining the contents of 16 ginsenosides from P. ginseng was established. Methods: The chromatographic separation was achieved on a C18 column (150 mm × 4.6 mm, 5 μm) using a mobile phase made up of acetonitrie and water at a flow rate of 1.0 mL/min. The detection wavelength and column temperature were set as 203 nm and 35°C, respectively. Results: Sixteen ginsenosides (Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3, F1, Rd, F2, Rg3, protopanaxatriol, compound K, Rh2, and protopanaxadiol) were separated at baseline with good linearity (r ≥ 0.9990). The recovery rates were 95%-102% (RSD < 2%). Conclusion: The method is simple, fast, accurate, and could be applied to the quality control of P. ginseng and its preparation.
