Quantitative analysis of calycosin-7-O-β-D-glucoside and formononetin in Astraglus membranaceus var. mongholicus by different processing methods
10.7501/j.issn.0253-2670.2015.11.022
- Author:
Yu-Ping SUN
1
Author Information
1. Tianjin University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Astraglus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao.;
Calycosin-7-O-β-D-glucoside;
Formononetin;
HPLC;
Processing methods
- From:
Chinese Traditional and Herbal Drugs
2015;46(11):1678-1681
- CountryChina
- Language:Chinese
-
Abstract:
Objective: An HPLC method was established to determine the content of calycosin-7-O-β-D-glucoside and formononetin in Astraglus membranaceus var. mongholicus. To study the influence of habitats, processing methods, and planting ages on the quality of A. membranaceus var. mongholicus. Methods: The HPLC condition was as follows: column: Dikma C18 (250 mm×4.6 mm, 5 μm); mobile phase: Acetonitrile-0.2% formic acid (gradient elution); flow speed: 1.0 mL/min; detection wavelength: 260 nm; temperature of column: 30℃. Results: Comparing the content of calycosin-7-O-β-D-glucoside and formononetin in 40 samples of A. membranaceus var. mongholicus from 11 different habitats, we could get the following: The processing methods of A. membranaceus var. mongholicus were cleaning and natural drying; Bayinnaoer city Wu Qianqi ming'an town head part was the best habitat for A. membranaceus var. mongholicus. The quality of A. membranaceus var. mongholicus cultivars grown for two years and three years had no difference. The quality of wild species was superior cultivars. Conclusion: The method is simple and rapid for determination, the contents of calycosin-7-O-β-D-glucoside and formononetin in A. membranaceus var. mongholicus. It can be used as a scientific and reliable basis for formulating the reasonable standard of cultivating and processing methods of A. membranaceus var. mongholicus.
