Nitrogeous chemical constituents from Tinosporae Radix
10.7501/j.issn.0253-2670.2015.09.006
- Author:
Ya-Ting SUN
1
Author Information
1. Key Laboratory of Structure-Based Drug Design & Discovery, School of Tractional Chinese Materia Medica, Shenyang Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
Alkaloid;
Echinuline;
Jatrorrhizine;
N-trans-feruloyltyramine;
Neoechinulin A;
Palmatine;
Tinosporae Radix
- From:
Chinese Traditional and Herbal Drugs
2015;46(9):1287-1291
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the chemical constituents from Tinosporae Radix (the roots of Tinospora sagittata) and their antiproliferative effects in cancer cells. Methods: The compounds were isolated and purified by means of silica gel, ODS, and Sephadex LH-20 column chromatography. The structures were identified on the basis of physicochemical properties and spectroscopic analysis. The antiproliferative effects in HL-60 and MCF-7 cells were tested by Trypan blue and MTT methods, respectively. Results: Nine compounds were isolated and their structures were identified as tetrahydropalmatine (1), tetrahydrojatrorrhizine (2), jatrorrhizine (3), palmatine (4), neoechinulin A (5), echinuline (6), N-trans-feruloyltyramine (7), uracil (8), and triethylamine hydroiodide (9). Conclusion: Among them, compounds 5, 6, 8 and 9 are isolated from the plants of Tinospora Miers for the first time. Compounds 1, 3-5 and 7 show the moderate antiproliferative effects in HL-60 cells.
