Study on purification technology of total flavonoids from Schizonepetae Herba with resistance of Caco-2 cells based on grey correlation degree analysis
10.7501/j.issn.0253-2670.2015.11.009
- Author:
Cong MA
1
Author Information
1. College of Pharmacy, Liaoning University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Caco-2 cells;
Grey correlation degree;
HPD-400 macroporous adsorption resin;
Purification process;
Total flavonoids of Schizonepetae Herba
- From:
Chinese Traditional and Herbal Drugs
2015;46(11):1615-1619
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Through the grey correlation analysis technology and the effect of Caco-2 colon cancer cell proliferation to evaluate the optimization of purification process in total flavonoids of Schizonepetae Herba by macroporous adsorption resin. Methods: By static adsorption and desorption rate of total flavonoids as examining indexes to screen the ratios of macroporous adsorption resin, using single factor experiment to optimize its purification process conditions of total flavonoids; The Caco-2 cell inhibition rate was determined by MTT method; At the same time, using the grey correlation degree analysis technology, the correlation between the purity of total flavonoids and proliferation inhibition rate of the tumor cell was calculated. Results: HPD-400 macroporous adsorption resin has the good adsorption separation performance of the total flavonoids from Schizonepetae Herba, Schizonepetae Herba at the concentration of 0.1 g/mL, 0.8 g/mL (medicine/wet resin) on the amount of column, using 2 BV water to remove impurity, eluting by 9 BV 70% ethanol and all of the flow rate are 4 BV/h, collecting the eluent and dry it; With the same method to do the secondary purification, the purity of total flavonoids is 92.15%, the inhibitory rate of Caco-2 cell proliferation is 86.17%, the greycorrelation coefficient of flavonoids purity and inhibition is 0.9502. Conclusion: This optimization process and the conditions are feasible and stable, the inhibitory efficacy of the in vitro Caco-2 cells is clearly. It lays the foundation of the new anti-cancer drugs based on the purification of total flavonoids in Schizonepetae Herba.
