HPLC method for simultaneous quantitative determination of six active ingredients in Rhodiola crenulata from different origins of Qinghai-Tibet Plateau
10.7501/j.issn.0253-2670.2015.02.023
- Author:
Qing LIU
1
Author Information
1. Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Catechin;
Ethyl gallate;
Gallic acid;
P-coumaric acid;
Rhodiola crenulata (Hook. f. et Thoms.) H. Ohba;
Salidroside;
Tyrosol
- From:
Chinese Traditional and Herbal Drugs
2015;46(2):276-279
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyze the six active ingredients in Rhodiola crenulata from the different origins of Qinghai-Tibet Plateau, to establish quantitative analysis methods, and to provide a basis for further use. Methods: Samples were extracted with 30% ethanol; In Diamonsil C18 column (250 mm × 4.6 mm,5 μm), gradient elution was carried out with acetonitrile -0.3% phosphoric acid solution as the mobile phase, detection wavelength 275 nm, and column temperature 25 ℃. Results: A good linear relationships of gallic acid, salidroside, tyrosol, catechin, ethyl gallate, and coumaric acid were at 38.2-382.0 (r = 0.9998), 301.0-3010.0 (r = 0.9999), 19.8 - 198.0 (r = 0.9996), 17.1-171.0 (r = 0.9996), 4.5-45.0 (r = 0.9998), and 6.38-63.80 ng/mL (r = 0.9994), respectively. Conclusion: The method is simple, rapid, accurate, and can be used simultaneously to determine the content of the six active ingredients in R. crenulata from the different origins of Qinghai-Tibet Plateau.
