Simultaneous determination of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose in Lygodii Herba by HPLC
10.7501/j.issn.0253-2670.2015.02.022
- Author:
Cai-Yi WANG
1
Author Information
1. Guilin Sanjin Pharmaceutical Co. Ltd.
- Publication Type:Journal Article
- Keywords:
6-O-p-coumaroyl-D-glucopyranose;
Caffeic acid;
Chlorogenic acid;
HPLC;
Lygodii Herba
- From:
Chinese Traditional and Herbal Drugs
2015;46(2):273-275
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for the determination of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose in Lygodii Herba. Methods: The determination was developed on Phenomenex C18 column (250 mm × 4.6 mm, 5 μm), methanol-acetonitrile-1% glacial acetic acid (5:5:90) was used as mobile phase, the detective wavelength was 325 nm, and the column temperature was 35 ℃. Results: The linear ranges of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose were 0.368-2.760, 0.064-0.480, and 0.102-0.765 μg, respectively. The average recoveries were 101.48% (RSD = 1.44%), 99.56% (RSD = 0.92%), and 101.73% (RSD = 1.62%), respectively. Conclusion: The method is simple, accurate, and highly reproducible, which could be used for the quality control of Lygodii Herba.
