Identification of donkey-hide and its counterfeits based on 1H-NMR metabolomics
10.7501/j.issn.0253-2670.2015.02.020
- Author:
Jun-Sheng TIAN
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, Shanxi University
- Publication Type:Journal Article
- Keywords:
Colla Corii Asini;
Counterfeit identification;
Donkey-hide;
Metabolomics;
NMR
- From:
Chinese Traditional and Herbal Drugs
2015;46(2):255-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The aqueous extracts of donkey-hide and its counterfeits (horse-hide and mule-hide) were identified using 1H-NMR metabolomics to provide the basis for the identification and quality control of donkey-hide gelatin. Methods: The water-soluble components of the hairless donkey-hide and its counterfeits were extracted and the 1H-NMR technique was applied to analyzing the components. Moreover, the NMR data were analyzed and the component belongings were recognized using relevant software for the multivariate statistical analysis. Results: Forty-two water-soluble components were identified in donkey-hide by 1H-NMR. There were more acetate, leucine, valine, isoleucine and less lactate, serine, pyroglutamate, and creatine in the horse-hide than in the donkey-hide; While there were more acetate, creatine, alanine, choline and less glycerol and acetate in mule-hide than in donkey-hide. And they were all in a significant difference (P < 0.05). Conclusion: The identification method of the raw material of Colla Corii Asini, namely, donkey-hide and its counterfeits based on 1H-NMR metabolomics was established for the first time, which provides the experimental references for the donkey-hide identification and quality control study.
