Cloning, bioinformatics, and expression analysis of sesquiterpene synthase gene As-SesTPS1 from Aquilaria sinensis
10.7501/j.issn.0253-2670.2015.05.020
- Author:
Xin HE
1
Author Information
1. Department of Pharmacy, Guangdong Pharmaceutical College
- Publication Type:Journal Article
- Keywords:
Aquilaria sinensis (Lour.) Gilg.;
Bioinformatics;
Clone;
Expression;
Sesquiterpene synthase
- From:
Chinese Traditional and Herbal Drugs
2015;46(5):733-739
- CountryChina
- Language:Chinese
-
Abstract:
To clone a sesquiterpene synthase gene denoted As-SesTPS1 from total RNA of Aquilaria sinensis based on previous transcriptome sequencing data and analyze the bioinformatics and expression of the gene. The full length cDNA of As-SesTPS1 was obtained by reverse transcription-PCR (RT-PCR). The sequence similarity and homology were analyzed, and the structure and function of the coding protein were predicted by bioinformatics method. The gene expression levels in different samples of A. sinensis were quantified using qRT-PCR technique. The sesquiterpene synthase gene As-SesTPS1 was cloned, which contained an ORF of 1 671 bp, encoding 556 amino acids, and shared high similarity to multiple sesquiterpene synthase genes. The protein encoded by this gene had conserved RRx8W and DDxxD motifs. It was confirmed the gene was highly expressed in agarwood sample through qRT-PCR technique. The sesquiterpene synthase gene As-SesTPS1 is cloned, which will lay a foundation for further study of sesquiterpene biosynthase pathway in A. sinensis.
