Correlation between contents of gastrodin and polysaccharides with grade of Gastrodia Tuber
10.7501/j.issn.0253-2670.2015.03.021
- Author:
You-Cheng LEI
1
Author Information
1. Key Laboratory of Standardization of Chinese Materia Medica Ministry of Education, School of Pharmacy, Chengdu University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Gastrodia Tuber;
Gastrodin;
Polysaccharides;
Quality assessment;
Quality evaluation
- From:
Chinese Traditional and Herbal Drugs
2015;46(3):418-423
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the correlation between the contents of gastrodin and polysaccharides with the variety of commercial specifications, grades, and cultivated areas of Gastrodia Tuber (Tianma), the tuber of Gastrodia elata, and then to choose the suitable chemical marker to assess the quality of Tianma raw materials. Methods: Gastrodin and polysaccharides were quantified in 35 Tianma samples including Winter Tianma which was harvested in winter, Spring Tianma which was harvested in spring with their different grades and cultivation areas by HPLC and spectrophotometry, respectively. Combined with the morphological characteristics, the correlation between the various Tianma samples with the amounts of gastrodin and polysaccharides was studied. Results: The content of polysaccharides in Winter Tianma (26.05%, n = 24) was higher than that in Spring Tianma (20.21%, n = 10). In contrast, the amount of gastrodin in Winter Tianma (0.49%, n = 24) was lower than that in Spring Tianma (0.68%, n = 10). Among Tianma samples with different grades, the content of polysaccharides in the first grade (32.65%, n = 6) and second grade (32.89%, n = 6) was higher than that in the third grade (21.71%, n = 8) and fourth grade samples (14.57%, n = 4). But, the amounts of gastrodin in the Tianma samples of the four grades were similar, which were 0.46% (n = 6), 0.65% (n = 6), 0.43% (n = 8), and 0.44% (n = 4) in the first, second, third, and fourth grade Tianma, respectively. Moreover, the contents of gastrodin and polysaccharides in Tianma samples collected from the famous cultivation regions such as Sichuan, Shaanxi, and Yunnan provinces of China were higher than those from others areas. Conclusion: The developed HPLC and spectrophotometry methods are accurate and reliable to assay the amounts of gastrodin and polysaccharides in Tianma. The amount of polysaccharides is related to the quality of Tianma samples authenticated by the commercial specifications, grades, and traditional experience, and therefore is a better marker to assess the quality of Tianma.
