Effect of D-α-tocopherol polyethylene glycol 1000 succinate on inhibition of MCF-7 cell proliferation by baohuoside I
10.7501/j.issn.0253-2670.2015.03.015
- Author:
Hong-Mei YAN
1
Author Information
1. Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Baohuoside I;
Breast cancer cell MCF-7;
Cell uptake;
Cytotoxicity;
D-α-tocopherol polyethylene glycol 1000 succinate
- From:
Chinese Traditional and Herbal Drugs
2015;46(3):384-388
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) on the inhibition of proliferation of breast cancer cells MCF-7 by baohuoside I. Methods: The cytotoxicity of baohuoside I to MCF-7 cells was determined by MTT assay, the cellular uptake of baohuoside I was detected by fluorescence microscopy, and the intracellular baohuoside I was determined by HPLC. Results: The effect of baohuoside I on the inhibition of MCF-7 cell proliferation was enhanced in the presence of TPGS, especially on lower concentration. The uptake rates of MCF-7 within 2 h were 29.51%, 38.12%, and 40.37%, when the proportions of baohuosaide I and TPGS were 1:1, 1:2, and 1:4, respectively. The ratios were increased by 27.92%, 65.24%, and 74.99% compared with those using baohuoside I only. Conclusion: TPGS can increase the uptake rate of baohuoside I in MCF-7 cells and enhance the inhibition of MCF-7 cell proliferation.
