Determination of loganin, paeoniflorin, and baicalein in Baogan Pills by RP-HPLC
10.7501/j.issn.0253-2670.2015.03.013
- Author:
Gen-Li HAN
1
Author Information
1. Department of Pharmacy, First Central Hospital
- Publication Type:Journal Article
- Keywords:
Baicalein;
Baogan Pills;
Loganin;
Paeoniflorin;
RP-HPLC
- From:
Chinese Traditional and Herbal Drugs
2015;46(3):374-377
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method of RP-HPLC for determining the mass concentration of loganin, paeoniflorin and baicalein in Baogan Pills, and it has provided the quality guarantee for the animal experiment which applied to the liver protection and reproductive function together with the basis of liver disease. Methods: Agilent Zorbax SB-C18 (150 mm × 46 mm, 5 μm) column was used. Acetonitrile and 0.1% phosphoric acid water-solution were used as mobile phase in gradient elution. The volume flow was at 1.0 mL/min; ultraviolent detection wavelength was 236 nm when loganin and paeoniflorin were detected, and was 278 nm when baicalein was detected; Column temperature was at 30 ℃ and injection volume was 5 μL. Results: The lowest detection limit in loganin was 14.3 μg/L, the linear range was 7.305-233.750 mg/L, and its RSD was 1.69%; The lowest detection limit in paeoniflorin was 30.9 μg/L, the linear range was 5.742-183.750 mg/L, and its RSD was 1.86%; The lowest detection limit in baicalein was 36.4 μg/L, the linear range was 3.711-118.750 mg/L, and its RSD was 1.76 %. The average recovery of loganin in Baogan Pills was 99.2%, and its RSD was 1.66%; The average recovery of paeoniflorin was 98.9%, and its RSD was 1.82 %; The average recovery of baicalein in was 100.6%, and its RSD was 1.90%. Conclusion: The method is believable for determining the mass concentration of loganin, paeoniflorin, and baicalein with its simplicity, sensibility, repeatability, and better recovery rate.
