Determination of related substances in Diammonium Glycyrrhizinate Enteric Coated Tablet by LC-MS
10.7501/j.issn.0253-2670.2015.03.011
- Author:
Xin JIANG
1
Author Information
1. Institute of Physical & Chemical Analysis, Jiangsu Provincial Center for Disease Prevention and Control
- Publication Type:Journal Article
- Keywords:
18α-glycyrrhizic acid;
Diammonium glycyrrhizinate;
Glycyrrhiza uralensis saponin;
Glycyrrhizin;
Impurities identification;
Licoricesaponine;
Ultra high performance liquid-diode array-ion trap-the time of flight tandem mass spectrometry
- From:
Chinese Traditional and Herbal Drugs
2015;46(3):365-368
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the methods for identifying the related substances in Diammonium Glycyrrhizinate Enteric Coated Tablet (DGECT) by ultra high performance liquid-diode array-ion trap-the time of flight tandem mass spectrometry (UPLC-DAD-IT- TOF-MS). Methods: The chromatographic separation was performed on an Agilent HC-C18 (250 mm × 4.6 mm, 5 μm) column, mobile phase was acetonitril-H2O-Hac (380:614:6), eluting sample was in equal degree flow, with volume flow rate of 1 mL/min, UV detection wavelength of 254 nm, and mass spectrum monitoring mass-to-charge ratio range m/z 100-1000. Results: Including licoricesaponine G2, H2, E2, B2, J2, C2, 18α-glycyrrhizic acid, and glycyrrhiza uralensis saponin (A or B), the eight kinds of related compounds existed were found. Conclusion: The related substances found in DGECT are valuable for the manufacturing process and quality control.
