Determination of seven components in flowers of Gentiana tibetica and Gentiana dendrologi by HPLC
10.7501/j.issn.0253-2670.2015.08.024
- Author:
Rong-Jiao LI
1
Author Information
1. Ethnic Pharmaceutical Institute, Southwest University for Nationalities
- Publication Type:Journal Article
- Keywords:
6′-O-β-D-glucopyranosyl gentiopicroside;
Gentiana dendrologi Marq.;
Gentiana tibetica King ex Hook;
Gentiopicroside;
HPLC;
Isoorientin;
Isovitexin;
Loganic acid;
Swertiamarin;
Swertiamarine
- From:
Chinese Traditional and Herbal Drugs
2015;46(8):1227-1230
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for simultaneously determining seven components, such as loganic acid, 6′-O-β-D-glucopyranosyl gentiopicroside, swertiamarine, gentiopicroside, swertiamarin, isoorientin, and isovitexin, from the flowers of Gentiana tibetica and G. dendrology. Methods: Chromatographic analysis was achieved on an Agilent Zorbax ODS column (150 mm × 4.6 mm, 5 μm) by gradient elution of acetonitrile-0.4% acetic acid in water at 30℃. The flow rate was 0.4 mL/min and the detection wavelength was 254 nm. Results: The calibration curves of all the seven constituents showed good linearity in a relatively wide concentration range. The linear ranges of loganic acid, 6′-O-β-D-glucopyranosyl gentiopicroside, swertiamarine, gentiopicroside, gentiopicroside, isoorientin, and isovitexin were 0.228-2.280, 0.680-6.800, 0.220-2.20, 1.476-14.760, 0.200-2.000, 0.436-4.360, and 0.276-2.760 μg (R2 ≥ 0.999 2), respectively. The recoveries (n = 9) of loganic acid, 6′-O-β-D-glucopyranosyl gentiopicroside, swertiamarine, gentiopicroside, gentiopicroside, isoorientin, and isovitexin were 100.9%, 99.4%, 101.0%, 105.7%, 103.1%, 98.4%, 104.2%. Conclusion: This method is simple, accurate, and specific, and can be used for the determination of seven constituents in the flowers of G. tibetica and G. dendrologi.
