Simultaneous determination of seven triterpenoid saponins in Ilex kudingcha with HPLC
10.7501/j.issn.0253-2670.2015.07.021
- Author:
Tao YANG
1
Author Information
1. School of Chinese Materia Medica, Guangzhou University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
HPLC;
Ilex kudingcha C. J. Tseng;
Kudinoside A;
Kudinoside D;
Kudinoside E;
Kudinoside G;
Latifoloside C;
Latifoloside G;
Latifoloside H
- From:
Chinese Traditional and Herbal Drugs
2015;46(7):1052-1055
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for simultaneously determining latifoloside G, latifoloside H, kudinoside G, latifoloside C, kudinoside A, kudinoside E, and kudinoside D in Ilex kudingcha. Methods: The separation was performed on a YMC C18 column (YMC-pack ODS-A, 250 mm × 4.6 mm, 5 μm) with water-acetonitrile as the mobile phase in a gradient elution at a flow rate of 1.0 mL/min. The detection wavelength was 210 nm and the column temperature was 30℃. Results: Seven triterpenoid saponins all have good separating degree, good linear range, and linearity (r > 0.999 5). The average sample recovery rates were 99.25%-100.8%, RSD < 2%. Conclusion: The established method is rapid, accurate, and with has repeatability and stability, which could provides the scientific evidence for the quality control of I. kudingcha.
