ISSR analysis of genetic background of triploid female and diploid male strains of Siraitia grosvenorii
10.7501/j.issn.0253-2670.2015.06.019
- Author:
Rong-Chang WEI
1
Author Information
1. Guangxi Botanical Garden of Medicinal Plants
- Publication Type:Journal Article
- Keywords:
Diploid;
Genetic background;
Inter simple sequence repeats;
Siraitia grosvenorii (Swingle) C. Jeffrey ex A. M. Lu et Z. Y. Zhang;
Triploid
- From:
Chinese Traditional and Herbal Drugs
2015;46(6):881-886
- CountryChina
- Language:Chinese
-
Abstract:
To evaluate the genetic background of triploid female and diploid male strains of Siraitia grosvenorii and provide the biological reference for good varieties breeding of seedless S. grosvenorii. Inter simple sequence repeats (ISSR) marker was developed to analyze the genetic background among 28 samples of S. grosvenorii, and cluster analysis and double principal coordinate analysis were revealed by the NTSYS-pc software and GenAIEx software, respectively. Out of 100 ISSR primers selected, 13 primers were used for amplification and a total of 131 unambiguous bands were obtained, among which 99 (PPB = 75.57%) were polymorphic. The results of cluster analysis and double principal coordinate analysis showed that there was a certain rich of genetic background in triploid female and diploid male strains of S. grosvenorii. But the genetic similarity coefficients of majority were bigger and the genetic distance was closer. The complexity of the genetic background in triploid female and diploid male strains of S. grosvenorii is lower and germplasm innovation strategies should be carried out to enrich the genetic background of the parents of seedless S. grosvenorii.
