Study on alcohol precipitation technology of Liuwei Dihuang Prescription
10.7501/j.issn.0253-2670.2015.08.012
- Author:
Ying-Chun WEI
1
Author Information
1. Jiangsu Kanion Pharmaceutical Co. Ltd.
- Publication Type:Journal Article
- Keywords:
Alcohol precipitation;
Liuwei Dihuang Prescription;
Pilot process;
Polysaccharide;
Production process
- From:
Chinese Traditional and Herbal Drugs
2015;46(8):1161-1166
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Through studying the rationalization of alcohol precipitation process of Liuwei Dihuang Prescription to obtain the stability alcohol precipitation process which could be used to promote industrialization. Methods: It screens the most reasonable alcohol precipitation process taking the contents, the relative molecular weight distribution, and the extract of loganin, peoniflorin, morroniside, manninotriose, and polysaccharide as the evaluation index to investigate the way of adding ethanol, centrifugation,, and whether to carry out the ethanol washing process. Results: The alcohol precipitation process is reasonable by calculating the alcohol volume method. The polysaccharides and alcohol precipitation supernatant could be efficiently separated by ethanol washing method. The polysaccharide extract and the contents of glycosides and manninotriose were up to the standard after the process improved. Conclusion: The optimized alcohol precipitation process is easy operation with good controllability and repeatability. It is worthy of popularization and application of industrialization.
