Preparation and characterization of baicalin-loaded polymeric micelles and its inhibition on MCF-7 cells
10.7501/j.issn.0253-2670.2015.04.009
- Author:
Wen-Zhuan MA
1
Author Information
1. Modern Research Center for Chinese Materia Medica, Beijing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Antitumor;
Baicalin;
Micelles;
MTT method;
Thin film hydration method;
TPGS
- From:
Chinese Traditional and Herbal Drugs
2015;46(4):507-512
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare baicalin-loaded TPGS nanomicells (BCN-TPGS-PMs) and to evaluate its physicochemical properties, in vitro release behavior, and antitumor activity against MCF-7 cells. Methods: BCN-TPGS-PMs were prepared by film-thin hydration method. The preparation methods and formulations were optimized and screened based on particle size and encapsulation efficiency (EE) of micelles. The transmission electron microscope (TEM) was used to observe the particle appearance, zetasizer instrument was used to detect the diameter and Zeta potential, and ultracentrifugation was utilized to determine the EE and drug-loading rate. Dynamic dialysis method was used to study the in vitro release behavior of BCN-TPGS-PMs, and the antitumor activity against MCF-7 cells was determined by MTT method. Results: The optimal BCN-TPGS-PMs were round with the nanometric size of (11.91 ± 0.14) nm, high EE rate of (95.83 ± 7.34)%, and drug-loading rate of (5.42 ± 0.04)%. The in vitro release behavior showed that BCN-TPGS-PMs had a slow release. Compared with free BCN, BCN-TPGS-PMs showed stronger cytotoxicity and inhibition against MCF-7 cells (P < 0.05). Conclusion: The prepared BCN-TPGS-PMs have small particle size, high drug-loading rate, and good stability, and could obviously increase the in vitro inhibitory effect of BCN.
