Identification of MicroRNAs from Eurya chinensis and function analysis on their target genes
10.7501/j.issn.0253-2670.2015.01.016
- Author:
Jie FANG
1
Author Information
1. Pharmacy College, Zhejiang Chinese Medical University
- Publication Type:Journal Article
- Keywords:
Eurya chinensis R. Brown;
High-flux sequencing;
MAPK signal pashway;
MicroRNAs;
Pharmacodynamic material
- From:
Chinese Traditional and Herbal Drugs
2015;46(1):80-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the pharmacodynamic MicroRNAs (miRNAs) in Eurya chinensis and the functions of their target genes. Methods: The miRNA profiles of E. chinensis in liver and plasma of normal mice and E. chinensis extract-treated mice were detected using high-flux sequencing, and then the miRNAs which took into the organism were validated by RTFQ-PCR using plant miRNA database in GeneBank. Human mRNA database as target was used to predict the target genes of miRNAs by TargetScan and MiRanda analysis, while the target gene functions were considered by GO and KEGG analyses. Results: Five pharmacodynamic microRNAs were identified and took part in biological processes, cellular components, and molecular functions, which affected tumor, metabolism, and inflammation-related signal pathways. Conclusion: The miRNAs might act as new pharmacodynamic materials of Chinese materia medica.