Effect of Leonuri Herba alkaloids on prostatic hyperplasia in older rats
10.7501/j.issn.0253-2670.2015.13.014
- Author:
Ming-San MIAO
1
Author Information
1. Henan University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Leonuri Herba alkaloids;
Male and female hormone induction;
Nutrilitr;
Older rat;
Prostatic hyperplasia
- From:
Chinese Traditional and Herbal Drugs
2015;46(13):1937-1943
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the pharmacological effects and mechanism of Leonuri Herba alkaloids (LHA) on prostate hyperplasia in older rats. Methods: The prostate hyperplasia model in older rats was induced by male and female hormone, the rats were divided into five groups: 50.0, 25.0, 12.5 mg/kg alkaloids dosage group, 30 mg/kg Longbishu Capsule suspension group, and model group given the same volume of saline. Otherwise aged rats and young rats were set as control groups administered with saline, and administered once daily for consecutive 30 d. The wet weight and prostate index in rat prostate were measured, and the expression of E2, DHT, T, bFGF, TGF-β1, EGF, and IGF-1 in serum and prostate tissue were detected after the experiment. The rat prostate tissue morphology changes and rat prostate cells ultrastructural changes were observed by light microscopy and transmission electron microscopy. Results: Compared with the model group, benign prostatic hyperplasia rat model was replicated successfully, low-dose LHA can significantly reduce the rat prostate wet weight and prostate index (P < 0.05), mid-dose LHA can significantly reduce the rat prostate index (P < 0.05). Each dose of LHA can significantly reduce the levels of T and DHT in prostate model rats (P < 0.01). Each dose of LHA can significantly reduce the expression of bFGF, EGF, and IGF-1 in prostate model rats (P < 0.01). Low-dose LHA can significantly increase the expression of TGF-β1 in prostate tissue. Each dose of LHA can significantly reduce the density of prostate model rats (P < 0.05, 0.01), and significantly increase the membrane surface and surface area values (P < 0.01). Each dose of LHA can significantly reduce intracytoplasmic mitochondria in prostate cells caused by model, and relieve the pathological changes of mitochondria cristae. Conclusion: LHA has good therapeutic effect to prostatic hyperplasia model rats induced by male and female hormone.
