Preparation and quality assessment of glycyrrhetinic acid-chitosan nanoparticles
10.7501/j.issn.0253-2670.2015.15.010
- Author:
Ya-Hui ZHANG
1
Author Information
1. College of Pharmacy, Gansu University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Chitosan;
Glycyrrhetinic acid;
Ionic cross-linking;
Nanoparticles;
Orthogonal design;
Quality assessment
- From:
Chinese Traditional and Herbal Drugs
2015;46(15):2232-2237
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the optimal preparation process of glycyrrhetinic acid-chitosan nanoparticles (GA-CS-NPs) and to evaluate the quality. Methods: The GA-CS-NPs were prepared by ionic cross-linking. The particle size, drug loading, and encapsulation efficiency were as evaluation indexes. The prescription and preparation process were optimized through single factor and orthogonal design. The quality of GA-CS-NPs was evaluated by morphology, particle size, drug loading, and encapsulation efficiency. Results: The optimal prescription was as follows: concentration of GA and CS was 0.2 and 2 mg/mL, ratio of CS and TPP (1.0 mg/mL) solutions was 20∶3. The mean diameter of GA-CS-NPs was (310.27 ± 10.02) nm, the entrapment efficiency and drug-loading efficiency were (51.42 ± 0.43)% and (6.87 ± 0.47)%. The evaluation showed that the appearance of GA-CS-NPs was round and uniform. It had a certain stability under lower temperature. Conclusion: The ionic cross-linking method used to prepare GA-CS-NPs is simple, rational, and stable.
