Effect of chlorogenic acid on carbohydrate metabolism in skeletal muscle of rats fed on high-fat diet
10.7501/j.issn.0253-2670.2015.17.013
- Author:
Bing-Jie PENG
1
Author Information
1. College of Bioscience & Biotechnology, Hunan Agriculture University
- Publication Type:Journal Article
- Keywords:
Carbohydrate metabolism;
Chlorogenic acid;
Glucose transporter;
Insulin;
Skeletal muscle
- From:
Chinese Traditional and Herbal Drugs
2015;46(17):2580-2585
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the mechanisms of chlorogenic acid (CGA) on regulating carbohydrate metabolism in skeletal muscle of SD rats fed on high-fat diet. Methods: Forty male SD rats were randomly divided into five groups: normal control (NC), high-fat diet (HFD), HFD with low-dose-CGA (20 mg/kg, HFD-LC), HFD with high-dose-CGA (90 mg/kg, HFD-HC), and Rosiglitazone (HFD-ROS) groups. NC rats were fed with normal chow diet, while the other rats were fed with HFD. The rats were killed after 12 weeks, and the real-time PCR method was used to measure the expression levels of GLUT-4, PKA, Akt, AMPKα2, IRS-1, PI3K, and PPARs mRNA in skeletal muscle. Results: The expression levels of GLUT-4, AMPKα2, and PPARβ in HFD group were more dramatically decreased than those in the NC group (P<0.05); Compared with the HFD group, CGA increased genes involved in carbohydrate metabolism, particularly Akt, PI3K, IRS-1, GLUT-4, PKA, AMPKα2, PPARα, and PPARβ in HFD-HC group (P<0.05). Conclusion: High-dose CGA could significantly slow the weight gain induced by HFD, as well as improve carbohydrate metabolism in skeletal muscle through regulating PI3K/Akt and AMPK signaling pathways, and insulin sensitivity.
