Mitigation effect of exogenous sucrose on root growth of licorice seedlings under salt stress conditions
10.7501/j.issn.0253-2670.2015.18.020
- Author:
Fu-Zhi LIU
1
Author Information
1. College of Agronomy, Gansu Agricultural University
- Publication Type:Journal Article
- Keywords:
CAT;
Exogenous sucrose;
Glycyrrhiza uralensis Fisch.;
Mitigation effect;
Salt stress;
SOD
- From:
Chinese Traditional and Herbal Drugs
2015;46(18):2781-2787
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the mitigation effect of exogenous sucrose on the root growth of licorice (Glycyrrhiza uralensis) seedlings under salt stress conditions. Methods: Licorice seedlings were taken as experimental materials, an experiment was conducted to study the effects of exogenous sucrose at different concentration on the growth and content of active ingredients, soluble sugar, proline, and activity of enzyme in licorice seedlings under NaCl stress conditions; Then to determine related indicators and analyze data by using the software SPSS 19.0. Results: This study showed that: Under salt stress conditions, after adding a certain concentration of exogenous sucrose, the relative growth rate of licorice seedlings per day was increasing, and this stress could be a significantly weakening effect, indicating that exogenous sucrose had salt stress-relieving effect. The total flavonoids and phenylalanine ammonia lyase (PAL) activity were significantly increased, the exogenous sucrose could mitigate the seedling roots under the salt stress, the licorice flavonoid content in the enhanced growth was largely, due to the activity of PAL, increased, when the concentration of exogenous sucrose was 10 mmol/L, PAL activity reaching a maximum; While the concentration of exogenous sucrose was 15 mmol/L, PAL activity turned into a downward trend, the results indicating that this mitigation had the effect depended on the concentration. After applying exogenous sucrose at different concentration, the content of licorice acid was higher than that under salt stress and reached the level without salt stress; The impact of exogenous sucrose at gradient concentration on licorice acid accumulation was not obvious. The content changes of proline and soluble sugar showed the correlation between the two. When 15 mmol/L of exogenous sucrose was applied both of them reached the controlled level; While the concentration of exogenous sucrose increasing on this basis, the contents of soluble sugar and proline in licorice seedlings were also on the rise. Compared with seedlings without salt stress treatment, the SOD and CAT activity in seedlings under the salt stress was increased; Certain concentration of exogenous sucrose could reduce the activity of SOD and CAT in seedlings under the salt stress. Conclusion: The exogenous sucrose may increase the activity of antioxidant enzymes in licorice seedlings, thereby maintaining a low level of reactive oxygen species in seedlings, and reducing the hurting degree of peroxide to cell, and improving the tolerance of licorice seedlings to the salt stress.
