Changes of alkaloids in Tibetan medicine Przewalskia tangutica during tissue culture and differentiation of its stem rapid propagation system
10.7501/j.issn.0253-2670.2015.20.021
- Author:
Tian-Xiang LEI
1
Author Information
1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences
- Publication Type:Journal Article
- Keywords:
Differentiation;
Inductivity;
Przewalskia tangutica Maxim.;
Tissue culture;
Tropane alkaloids
- From:
Chinese Traditional and Herbal Drugs
2015;46(20):3086-3090
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the stem rapid propagation culture system of Prezewalskia tangutica using aseptic seedling of P. tangutica as materials. Methods: The stems from one month aseptic seedling were inoculated on Muragshige and Skoog media supplemented with different phytohormones to select the optimum medium for inducing differentiation of the adventitious buds and roots. Furthermore, the contents of four active alkaloids were measured with HPLC. Results: The optimum medium was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L for inducing the propagation of the adventitious buds. The adventitious roots could be induced on MS+NAA 0.5 mg/L media. The inductivity was 71.67%. The HPLC data suggested that the content of total alkaloids is highest in root (141.25 μg/g). The content of total alkaloids in callus was lower than those in roots, stems, and leaves. Conclusion: The contents of total alkaloids in different fractions of P. tangutica seedlings are different, and the proportion of different alkaloids in total alkaloids is different, which indicates that the formation and dedifferentiation of organ can influence the contents of alkaloids in P. tangutica seedlings.
