Quality assessment of Longxue Tongluo Capsule based on HPLC fingerprint and multi-components simultaneous determination
10.7501/j.issn.0253-2670.2015.20.011
- Author:
Jian-Ping QIN
1
Author Information
1. Jiangsu Kanion Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
2,6-dimethoxy-4,4'-dihydroxydihydrochalcone;
2-methoxy-4,4'-dihydroxydihydrochalcone;
7,4'-dihydroxy flavone;
Fingerprint;
HPLC;
Longxue Tongluo Capsule;
Loureirin A;
Loureirin B;
Multi-components determination;
Pterostilbene;
Resveratrol
- From:
Chinese Traditional and Herbal Drugs
2015;46(20):3028-3033
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC fingerprint of Longxue Tongluo Capsule (LTC), to determine the multi-components in LTC, and to provide the basis for the evaluation of LTC. Methods: The Kromasil C18 (250 mm×4.6 mm, 5 μm) column was used with a mobile phase of acetonitrile-0.1% formic acid gradient elution, the flow rate was 1.0 mL/min, the column temperature was 35℃, and the detection wavelength was 280 and 325 nm. Ten batches of LTC were detected to eatablish fingerprints, the common peaks were identified by Q-TOF/MS, and some of the characteristic peaks were analyzed. Results: The fingerprint chromatography with good resolution and reproducibility included 18 mutual peaks, and the similarity was more than 0.92.Sixteen common peaks had been identified by LC-Q-TOF/MS, seven of which were unequivocally identified via comparing the retention time, mass spectra, and MS/MS spectra data with those of the standard compounds. Then the seven maker components were identified as resveratrol, 7,4'-dihydroxy flavone, loureirin A, loureirin B, pterostilbene, 2,6-dimethoxy-4,4'-dihydroxydihydrochalcone, and 2-methoxy-4,4'-dihydroxydihydrochalcone. The seven components were quantified, and the recoveries ranged from 98.47% to 101.93% with RSD values of less than 2.27%. Conclusion: The method is rapid, simple, and accurate, and can be used for the quality control of LTC.
