Study on colonization of endophytic antagonistic strain JYg07 of Schisandra chinensis
10.7501/j.issn.0253-2670.2015.21.021
- Author:
Yan JIN
1
Author Information
1. College of Agronomy, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Antibiotic-resistance tag;
Colonization;
Endophytic antagonistic bacteria;
Schisandra chinensis (Turcz.) Baill;
Strain JYg07
- From:
Chinese Traditional and Herbal Drugs
2015;46(21):3242-3247
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The colonization of endophytic antagonistic bacteria in different parts of Schisandra chinensis was tested in the meantime. Methods: Using antibiotic notation method, mutant strains of JYg07 resistant to streptomycin and rifampin at the same time were obtained. To test the colonization the labeled strains of JYg07 with different vaccination methods of root irrigation, spray, injection, and stabbing leaves. Results: Through the natural orifice and the wound, the strain JYg07 could enter the plant of S. chinensis and long live in soil. Bacterial liquid concentration influenced the time of colonization peak. Using four vaccination methods, the labeled strains of JYg07 could colonize and engraft rapidly in S. chinensis, and the colonization ability in the roots was higher than that in the stems and leaves. High concentration of nutrient solution method and root irrigation method could make JYg07 in the best colonization state in S. chinensis. Conclusion: Strain JYg07 could colonize and engraft rapidly in S. chinensis, which could be developedt and utilized as new resources of biocontrol bacteria.
