Protection of harpagide on cytotoxicity in PC12 cells induced by Aβ25-35 peptides
10.7501/j.issn.0253-2670.2015.23.016
- Author:
Fei WANG
1
Author Information
1. Fuzhou Medical College of Nanchang University
- Publication Type:Journal Article
- Keywords:
Amyloid-β;
Apoptosis;
Harpagide;
Oxidative stress;
PC12 cells
- From:
Chinese Traditional and Herbal Drugs
2015;46(23):3539-3543
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The main purpose of the present study is to investigate the influence of harpagide on amyloid-β25-35 (Aβ25-35)- induced cytotoxicity. Methods: Oxidative stress was assessed by measuring MDA, glutathione (GSH), and superoxide dismutase (SOD) levels. Cell viability was assessed by MTT assay. Cell apoptosis detection was performed using an Annexin-V-FITC Apoptosis Detection Kit. The production of ROS was determined using a ROS Assay Kit. Western blotting detection was carried out to detect the protein expression. Results: Our studies showed that pretreatment with harpagide could reduce Aβ25-35-induced oxidative stress. Harpagide markedly inhibited cell apoptosis in a dose dependent manner. More importantly, harpagide increased the PI3K/AKt and Bcl-2 family protein ratios on pre-incubation with cells for 3 h. Conclusion: One possible mechanism of harpagide to improve Aβ25-35-induced PC12 cytotoxicity may be through the up-regulation of SOD and activation of the PI3K/AKt pathway.
