Analysis on chemical constituents from flowers of Trollius ledebouri by UPLC-Q-TOF-MS
10.7501/j.issn.0253-2670.2015.23.006
- Author:
Xiao-Lei REN
1
Author Information
1. Insititute of Chinese Materia Medica, Heilongjiang Academy of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Chemical constituents;
Flavone;
Flowers of Trollius ledebouri;
Orietin;
UPLC-Q-TOF-MS;
Vanillic acid;
Vevatric acid
- From:
Chinese Traditional and Herbal Drugs
2015;46(23):3475-3481
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The chemical constituents in the methanol extract from the flowers of Trollius ledebouri were rapidly identified using UPLC-Q-TOF-MS in positive and negative ion modes. Methods: The analysis was performed on an ACQUITY UPLC BEH C18 reverse phase column (100 mm × 2.1 mm, 1.7 μm). The mobile phase consisted of methanol and 0.1% formic acid was used for gradient elution, 0-5 min, 95%-60% A; 5-13 min, 60%-45% A; 13-20 min, 45%-0% A; 20.1-25 min, 95% A. The flow rate was 0.3 mL/min, the injection volume was 2 μL. The information of the compounds was analyzed by the positive and negative ion mass spectra and elements composition. Results: The results indicated that 14 compounds in the extract from the flowers of T. ledebouri had been identified by direct comparison in both positive and negative ion mass data, they were vevatric acid, vanillic acid, orietin, orietin-2″-O-β-L-galactopyranoside, orietin-2″-O-β-D-xylopyranoside, quercetin, hyperoside, vitexin, apigenin, luteolin, acacetin-7- O-neohesperidoside, acacetin, pectolinarigenin, and salvigenin. Conclusion: UPLC-Q-TOF-MS could be used to analyze the constituents in the extract from flowers of T. ledebouri and to provide the protection for the quality control of the pieces as well as the stability and efficacy in the late stage clinical application, and to provide a reference for clarifying the material basis of its efficacy.
