Determination of eight components in Qibai Pingfei Granule by quantitative analysis of multi-components with a single-marker
10.7501/j.issn.0253-2670.2015.24.012
- Author:
Rui-Ting JIN
1
Author Information
1. Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Astragaloside;
Ginsenoside Rb1;
Ginsenoside Rb2;
Ginsenoside Rc;
Ginsenoside Rd;
Ginsenoside Re;
Ginsenoside Rf;
Ginsenoside Rg1;
HPLC-ELSD;
Qibai Pingfei Granule;
Quantitative analysis of multi-components with a single-marker;
Relative correlation factors;
Saponins
- From:
Chinese Traditional and Herbal Drugs
2015;46(24):3682-3686
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a quantitative analysis of multi-components with a single-marker (QAMS) method for the determination of eight components in Qibai Pingfei Granule (QPG). Methods: Ginsenoside Rb1 was used as the internal reference substance. The relative correlation factors (f) of ginsenosides Rg, Re, Rf, Rc, Rb2, Rc, and astragaloside were calculated and established. The results were compared with those obtained by the external standard method in 10 batches of QPG. The results showed that the f values of ginsenosides Rg, Rf, Rc, Rb2, astragaloside, and ginsenosides Rc to ginsenoside Rb1 were 1.244, 1.075, 1.133, 1.090, 1.071, 0.967 and 1.070. Results: Ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, Rd, and astragaloside had good relations within the ranges of 0.416 0-4.992, 0.315 4-3.785 2, 0.259 6-3.115, 0.385 2-4.622, 0.222 8-2.674, 0.193 2-2.318, 0.183 5-2.202, and 0.574 6-6.895 μg, respectively. The two methods did not show the significant difference in assay results. Conclusion: So the QAMS method is feasible and credible, and could be used to determine the multiple components in QPG.
