Chemical constituents of triterpenoids from Prunella vulgaris and their antitumor activities
10.7501/j.issn.0253-2670.2015.24.002
- Author:
Yu-Bing BAI
1
Author Information
1. Hunan University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
3-hydroxy-11-en-11,12-dehydrogenation-28,13-oic acid lactone;
Betulic acid;
Breast cancer cell;
Eburicol;
Prunella vulgaris L.;
Triterpenoids
- From:
Chinese Traditional and Herbal Drugs
2015;46(24):3623-3629
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the chemical constituents from Prunella vulgaris and their antitumor activities. Methods: Silica gel, reverse-phase octadecylsilyl (ODS), Sephadex LH-20 chromatographic methods, and HPLC were applied to isolating and purifying compounds. MS and NMR spectroscopic methods were used to determine their structures. Furthermore, the cytotoxicity of these chemical components for MCF-7, MDA-MB-231, and MCF-10A cell lines was measured by MTT method. Results: Forteen compounds were isolated from the fruits of P. vulgaris and their structures were identified as: autantiamide acetate (1), 5α,8α-epidioxy-(22E,24R)-ergosta-6,22-dien-3β-ol (2), β-amyrin (3), betulic acid (4), 3-hydroxy-11-en-11,12-dehydrogenation-28,13-oic acid lactone (5), eburicol (6), 2α,3α,24-trihydroxyolean-12-en-28-oic acid (7), candelabrone 12-methyl ether (8), cyclopentaneacetic acid (9), 2α,3β-dihydroxyursa-12-en-28-oic acid (10), α-spinasterol (11), oleanolic acid (12), ursolic acid (13), and β-sitosterol (14). Conclusion: Compounds 2, 5, 6, 8, and 9 are isolated from the genus of Prunella L. for the first time. The results of cytotoxic assay indicate that compounds 10 and 13 can obviously inhibit the activity of MCF-7, MDA-MB-231, and the normal cell lines MCF-10A. Compound 4 can selectly inhibit the activity of MCF-7 and MDA-MB-231 while show no effect on the normal cell lines MCF-10A.
