Protection of 20 Chinese materia medica extracts to Aβ25-35-induced SH-SY5Y cell injury using high content screening
10.7501/j.issn.0253-2670.2016.02.015
- Author:
Yan-Ru WANG
1
Author Information
1. Jiangsu Kanion Parmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
Apoptosis;
Aβ25-35;
Chinese materia medica extracts;
High content screening;
SH-SY5Y cell
- From:
Chinese Traditional and Herbal Drugs
2016;47(2):267-274
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify neuroprotective extracts with the protective effects on Aβ25-35-induced SH-SY5Y cell injury via high content screening (HCS). Methods: Hoechst 33342/PI double staining method was used to screen neuroprotective extracts from 60 Chinese materia medica (CMM) extracts. Further more, the effects of neuroprotective extracts on Aβ25-35-induced changes in the levels of Caspase-3/7 were detected. Results: The results showed that 17 extracts had obviously neuroprotective effects. Among the 17 extracts, 8 of them inhibited Aβ25-35-induced up-regulation of Caspase-3/7. Conclusion: HCS is an efficient method to screen neuroprotective extracts with the protective effects of Aβ25-35-induced SH-SY5Y cell injury. The neuroprotective extracts have potential medicinal value in Alzheimer's disease.
