Major phenolic acids in Usneae Filum by UHPLC-Triple-TOF-MS
10.7501/j.issn.0253-2670.2016.03.007
- Author:
Ying-Hua MA
1
Author Information
1. Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University
- Publication Type:Journal Article
- Keywords:
Diffractaic acid;
Orsellinic acid;
Phenolic acids;
UHPLC-Triple-TOF-MS;
Usneae Filum;
Usnic acid
- From:
Chinese Traditional and Herbal Drugs
2016;47(3):392-400
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a quick method of ultra-performance liquid chromatography-quadrupole time-of-fight mass spectrometry (UHPLC-Triple-TOF-MS) for the identification of phenolic acids (diffractaic acid, usnic acid, orsellinic acid, etc.) in Usneae Filum. Methods: The separation was performed on the chromatographic column of Phenomenex Luna 3u C18 (150 mm × 2.0 mm, 3 μm), and the mobile phase was methanol (0.05% formic acid)-0.05% formic acid solution (4 mmol ammonium acetate), with a gradient elution at a flow rate of 0.3 mL/min. The column temperature was 30℃, and negative ion mode was used for TOF-MS. Results: Seventeen compounds were identified or tentatively characterized based on the retention time and MS spectra. They are depsides, two benzo furan, ant multi substituted single benzene as well. And the preliminary fragmentation rules are summarized. Conclusion: The results demonstrate that UHPLC-Triple-TOF-MS method is novel, quick, and efficient for the identification of the compounds in Usneae Filum.
