Research on separation and purification of extract from Dracocephalum moldevica with macroporous resins
10.7501/j.issn.0253-2670.2016.04.012
- Author:
Ning YU
1
Author Information
1. Xinjiang Institute of MeteriaMedica
- Publication Type:Journal Article
- Keywords:
Dracocephalum moldavica L.;
Luteolin-7-O-β-D-glucuronide;
Macroporous adsorption resins;
Rosmarinic acid;
Tilianin;
Total flavonoids
- From:
Chinese Traditional and Herbal Drugs
2016;47(4):599-605
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the technology for the separation and purification of extract in Dracocephalum moldevica (EDM) by macroporous resin. Methods: Static and dynamic adsorption-desorption were used to select the best one from seven different type macroporous resins; With the content of total flavonoids, tilianin, luteolin-7-O-β-D-glucuronide, and rosmarinic acid as indexes, the purification technology parameters of EDM were optimized. Results: HPD600 resin showed the best purifying profile, its optimum technology conditions were as follows: The optimum concentration of the sample liquid was 0.08 g/mL equivalent to raw material, the resin column diameter-height ratio was 1:9, the amount of used adsorption was 0.32 g dried medicinal herb/mL resin, sample flow rate was 1.5 BV/h, and adsorption time was 12 h. In the course of elution, the resin column chromatography was eluted with 6 BV of 70% ethanol after removing impurities with 4 BV of water by flow rate of 1.5 BV/h. The contents of total flavonoids, tilianin, luteolin-7-O-β-D-glucuronide, and rosmarinic acid were more than 53%, 5.5%, 4.7%, and 2.5%. Conclusion: Macroporous resin HPD600 is suitable to separate and purify EDM.
