Study on capillary GC fingerprint of Guominxing Biyan Granules
10.7501/j.issn.0253-2670.2016.06.011
- Author:
Jun-Hua HU
1
Author Information
1. Jiangsu Kanion Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Capillary GC;
Fingerprint;
GC-MS;
Guominxing Biyan Granules;
Similarity
- From:
Chinese Traditional and Herbal Drugs
2016;47(6):923-927
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the capillary GC fingerprint of Guominxing Biyan Granules and give a new method for quality control. Methods: The capillary GC was used. The ingredients of common peaks were analyzed by FID and MS. The HP-5 Phenyl Methyl Siloxane (30 m×0.32 mm, 0.25 μm) was used for capillary column. The chromatography conditions were as follows: the temperature of inlet was 200℃ in splitless sampling, the detector temperature was 250℃, the column initial temperature was 50℃, 3℃/min rose to 140℃, keeping 2 min and 10℃/min rose to 200℃, keeping 2 min. Results: Capillary GC fingerprint of volatile oil in Allergic Rhinitis Particles was established. Twelve peaks were selected as the fingerprint peaks of Allergic Rhinitis Particles. The ingredients of common peaks were designated by GC-MS. The similarity between the fingerprint of 10 batches of Allergic Rhinitis Particles Samples were 0.90. Conclusion: The established capillary GC fingerprint has desirable precision, reproducibility, and stability, and can be applied to the quality control of Guominxing Biyan Granules.
