Preliminary evaluation on drug release and local security of Ranuncoli Ternati Radix extracts-ion sensitive in situ gels
10.7501/j.issn.0253-2670.2016.07.017
- Author:
Chun-Hui HU
1
Author Information
1. Department of Pharmacy, Medical College of Qinghai University
- Publication Type:Journal Article
- Keywords:
Alveolar;
Fluorescence in vivo imaging system;
Interventional therapy;
Ion sensitive in situ gels;
lavage technique;
Ranuncoli Ternati Radix;
Safety in pulmonary
- From:
Chinese Traditional and Herbal Drugs
2016;47(7):1163-1168
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the pulmonary retention, safety, and local drug concentration of Ranuncoli Ternati Radix extracts- ion sensitive in situ gels (RTRE-ISG). Methods: Sodium alginate as excipient, RTRE (70% ethanol) as solvent, it was swollen at room temperature using magnetic stirring to prepare RTRE-ISG which was administered via endotracheal tube to rats, The levels of inflammatory cytokines and fibrosis factors were detected in BALF to screen the relative safe priscription. Then using CY5.5 as fluorescent dye, the pulmonary retention of RTRE-ISG was evaluated with the aid of the small animal in vivo imaging system. In the end, the lung lavage was performed by using the improved alveolar lavage technique and the local drug concentration was determined. Results: The safety of RTRE-ISG prepared by 1% sodium alginate was better, the retention time in lung was 120-144 h revealed by fluorescence in vivo imaging system. The results showed that the release of drug reached to a peak of 5.24 μg/mL in 5 h after administration and 0.08 μg/mL in 144 h after administration. Conclusion: RTRE-ISG was good safety with the retention time in lung more than 120 h, which is a nevol sustained preparation of Chinese materia medica for the treatment of pulmonary tuberculosis.
