HPLC fingerprint of Guanxin Shutong Capsule
10.7501/j.issn.0253-2670.2016.07.013
- Author:
Fei-Fei ZHOU
1
Author Information
1. School of Pharmacy, Shenyang Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
Cryptotanshinone;
Danshensu;
Dihydrotanshinone I;
Ellagic acid;
Eugenol;
Fingerprint;
Gallic acid;
Guanxin Shutong Capsule;
HPLC;
Procatechuic aldehyde;
Protocatechuic acid;
Quality control;
Rosmarinic acid;
Salvianolic acid A;
Salvianolic acid B;
Tanshinone I;
Tanshinone IIA
- From:
Chinese Traditional and Herbal Drugs
2016;47(7):1137-1141
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC method for fingerprint analysis of Guanxin Shutong Capsule (GSC) for its quality control. Methods: The chromatographic behaviors were obtained by an Agilent TC-C18 column (250 mm × 4.6 mm, 5 μm) with gradient elution using 0.05% phosphoric acid and acetonitrile as the mobile phase. The column temperature was set at 30 ℃ and the flow rate was 1.0 mL/min. The detection wavelength was set at 280 nm. The common mode of HPLC fingerprint for 10 batches of GSC was established with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2009 edition) and the common peaks were identified by reference compounds. Results: Fingerprints of 10 batches of GSC were established and the similarities to the common mode were above 0.95. Totally 45 common peaks were found, and 34 belonged to herbs. Five mutual peaks were from Choerospondias axillaris, 23 mutual peaks were from Salvia miltiorrhiza, and seven mutual peaks were from Syzigium aromaticum. Based on the retention time of reference substances, 13 constituents including gallic acid (peak 1), danshensu (peak 3), protocatechuic acid (peak 5), procatechuic aldehyde (peak 6), ellagic acid (peak 10), rosmarinic acid (peak 16), salvianolic acid A (peak 19), salvianolic acid B (peak 23), eugenol (peak 24), dihydrotanshinone I (peak 30), cryptotanshinone (peak 34), tanshinone I (peak 35), and tanshinone IIA (peak 39) were identified. Conclusion: This method is specific and reliable, which will be conducive to the quality control of GSC.
