Identification of Bupleuri Radix and its adulterants by DNA barcoding
10.7501/j.issn.0253-2670.2016.09.023
- Author:
Xiao-Wei HAN
1
Author Information
1. Hebei University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Barcoding;
Bupleuri Radix;
Chinese medicinal materials;
Identification;
ITS2 sequence
- From:
Chinese Traditional and Herbal Drugs
2016;47(9):1583-1588
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the authenticity of Bupleuri Radix in the market of Hebei province. Methods: In this research, the ITS2 sequence of 28 samples of Bupleuri Radix that were bought from the market in Hebei province was amplified by PCR, and the sequence of seven samples was downloaded from GenBank. Aligner CodonCode software was used for sequence alignment, the MEGA6.0 analysis was used to calculate the K2P distance, and the mutation sites of each sequence was analyzed. Results: Among the 28 Bupleuri Radix samples, there were 21 samples for Bupleurum chinese, 3 for B.smithii, and 4 for the false. Conclusion: ITS2 sequence can be used as an effective method to identify the authenticity of Bupleuri Radix, it is great value to the medicine market standard and the clinical medication safety.
