Effect of Xiaoyan Decoction on bone marrow suppression of mice with Lewis lung cancer
10.7501/j.issn.0253-2670.2016.09.020
- Author:
Pei-Ying YANG
1
Author Information
1. Tianjin University of Traditional Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Bone marrow suppression;
Chemotherapy;
EPO;
GM-CSF;
IL-3;
Immune function;
Lewis lung cancer;
Xiaoyan decoction
- From:
Chinese Traditional and Herbal Drugs
2016;47(9):1567-1571
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effects and mechanism of Xiaoyan Decoction on bone marrow suppression in mice from aspects of peripheral blood cell ratio, immune function, and cytokines. Methods: A mouse model of bone marrow suppression was established. Fifty mice were randomly divided into five groups (n = 10): control group, model group, Xiaoyan Decoction group, rhG-CSF group, and Xiaoyan Decoction combined with rhG-CS group. Lewis-tumor mice in Xiaoyan Decoction group were ig given Xiaoyan Decoction 18.2 g/(kg·d) twice daily, for 7 d. Lewis-tumor mice in rhG-CS group were treated by sc injection of rhG-CSF. The routine blood test, immunological functions of peripheral blood and cytokines (IL-3, IL-6, EPO, and GM-CSF) in serum were detected. Results: The bone marrow suppression model was successfully established. Compared with control group, the levels of WBC, RBC, Hb, and PLT in peripheral blood of model group were decreased, especially in WBC (P < 0.05). There is a remarkable increase in the level of WBC in Xiaoyan Decoction group and rhG-CS group as compared with model group (P < 0.05). Contrasted with model group, a significant increasing of CD3+CD8+T cells and CD3+CD8+cells on peripheral blood lymphocytes was observed in Xiaoyan Decoction group and combined group. The level of Treg cell in combined group was significantly less than that of Treg cell in rhG-CS group (P < 0.05). And there was no difference in NK cell level among all these groups (P > 0.05). The concentration of IL-3 and IL-6 was dramatically reduced in model group compared with control group (P < 0.05). And it showed an obviously increasing trend in IL-3, IL-6, EPO, and GM-CSF levels in Xiaoyan Decoction group and combined group, as well as an increase in IL-3, IL-6 levels in rhG-CS group (P < 0.05). Conclusion: It shows that Xiaoyan Decoction combined with rhG-CS can improve the level of WBC and immunological functions of peripheral blood in bone marrow suppression model mice. And the effect of combination of both drugs is more significant. This might be related to the effect of promoting the production of serum hematopoietic regulator such as IL-3, IL-6, EPO, and GM-CSF, and relieving chemotherapy-induced bone marrow suppression.
