Establishment of callus culture system of Tribulus terrestris and determination of total flavonoid glycosides
10.7501/j.issn.0253-2670.2016.10.023
- Author:
Ying DAI
1
Author Information
1. College of Chinese Medicinal Materials, Jilin Agricultural University
- Publication Type:Journal Article
- Keywords:
Callus;
Flavonoid glycosides;
Secondary metabolite;
Tissue culture;
Tribulus terrestris L.
- From:
Chinese Traditional and Herbal Drugs
2016;47(10):1768-1774
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the callus culture system of Tribulus terrestris and investigate the contents of the total flavonoid glycosides in its seeds, whole herb, and callus. Methods: By using the orthogonal test design, different explants, the effects of culture medium, light condition, suitable hormones, and their ratio on callus induction and proliferation were studied. Callus culture system of T. terrestris was determined and the total flavonoid glycosides of its seeds, whole herb, and callus were detected. Results: The stems of T. terrestris were used as the experimental materials. The optimal medium for the callus induction was MS + 2,4-D 0.5 mg/L + 6-BA 0.1 mg/L, with temperature of 25 ℃ and darkness of 24 h/d. The optimal medium for callus differentiation was MS + 2,4-D 1.0 mg/L + 6-BA 0.2 mg/L + ZT 1.0 mg/L, with light of 12 h/d, pH of 6.2, and subculture cycle of 35 d. The total flavonoid glycosides in the seeds, whole herb, and callus of T. terrestris were 1.46%, 2.01%, and 5.22%. Conclusion: Callus induced by tissue culture technique are soft, luster, and transparent, which are suitable for establishing the stable and efficient callus culture system of T. terrestris. The total flavonoid glycosides in callus are more than those in the seeds and whole herb.
