Inhibitory effect and mechanism of galangin on breast cancer metastasis
10.7501/j.issn.0253-2670.2016.10.017
- Author:
Yi-Fu XU
1
Author Information
1. Department of Pharmacy, First Affiliated Hospital of Soochow University
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Galangin;
Invasion;
MDA-MB-231;
Migration;
MMPs
- From:
Chinese Traditional and Herbal Drugs
2016;47(10):1731-1739
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of galangin on the migration and invasion of breast cancer and its action mechanism. Methods: MTT assay was adopted to observe the effect of galangin at different concentration on the growth capacity of breast cancer cells MDA-MB-231. Wound healing and Transwell assay were conducted to detect the effect of galangin on the migration and invasion of MDA-MB-231 cells. Western blotting and immunofluorescence assay were adopted to investigate the migration and invasion related proteins including PI3K, AKT, MMP-2, and MMP-9. The effect of galangin on NF-κb p65 phosphorylation and NF-κb p65 nuclear transition in vitro was assayed by Western blotting and immunofluorescence assay. Furthermore, the antitumor effect of galangin in MDA-MB-231 tumor bearing BALB/c mice model was assayed in vivo. Results: Galangin inhibited the migration of MDAMB-231 cells in a dose-dependent manner. In wound healing and transwell experiments, with the increase in the concentration of galangin, the number of migrant MDA-MB-231 cells and the invasion capacity of breast cancer cells decreased. Galangin could decrease the protein expression of PI3K, Akt, MMP-2, and MMP-9. Besides, galangin significantly inhibit the phosphorylation of transcription factor NF-κB p65 and NF-κB p65 nuclear transition. Galangin also inhibited the growth of MDA-MB-231 cancer cells in vivo and down-regulated PI3K, Akt, MMP-2, and MMP-9. Conclusion: Galangin could inhibit the growth capacity of breast cancer cells MDA-MB-231 and block the migration and invasion of MDA-MB-231 cells. The mechanism for antitumor effect of galangin is through inhibiting NF-κb p65 activity and MMP-2/9 expression, and blocking PI3K-AKT signaling pathway.
