Contents of seven indicative constituents in seven kinds of Gentiana macrophylla by UPLC
10.7501/j.issn.0253-2670.2016.11.026
- Author:
Yan-Mei YANG
1
Author Information
1. Gansu University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
6'-O-β-D-glucosyl gentiopicroside;
Gentiana macrophylla Pall.;
Gentiopicroside;
HPLC;
Isoorientin;
Isovitexin;
Loganic acid;
Sweroside;
Swertiamarin;
UPLC
- From:
Chinese Traditional and Herbal Drugs
2016;47(11):1968-1973
- CountryChina
- Language:Chinese
-
Abstract:
Objective: An ultra performance liquid chromatographic (UPLC) method was developed for simultaneously determining seven constituents, such as loganic acid, swertiamarin, 6'-O-β-D-glucosyl gentiopicroside, gentiopicroside, sweroside, isoorientin, and isovitexin, from the medicinal plants of Gentiana macrophylla. Methods: The separation was performed on an Acquity UPLC® BEH C18 column (50 mm ×2.1 mm, 1.7 μm) through a gradient elution of methanol-0.04% aqueous phosphorite at a flow rate of 0.3 mL/min. The detection wavelength was 242 nm, and the column temperature was set at 30℃. Results: For the seven analytes, loganic acid, swertiamarin, 6'-O-β-D-glucosyl gentiopicroside, gentiopicroside, sweroside, isoorientin, and sovitexin, a good linearity (r≥0.9995) was obtained in the range of 2.100-537.100, 1.050-270.000, 0.920-236.000, 11.100-2830.000, 0.750-192.000, 0.167-102.000, and 0.216-52.800 μg, respectively. Their average recoveries (n=6) were 97.83%-100.08%, respevtively, with RSD values less than or equal to 3.76%. Conclusion: The UPLC method is simpler and more effective than HPLC, and can be used for the simultaneous determination of seven indicative constituents in medicinal plants of G. macrophylla.
