Separation and purification of emodin-8-O-β-D-glucopyranoside from Polygoni Cuspidati Rhizoma et Radix by column and high-speed counter-current chromatography
10.7501/j.issn.0253-2670.2016.12.017
- Author:
Dong-Mei ZHAO
1
Author Information
1. School of Pharmacy Qingdao University
- Publication Type:Journal Article
- Keywords:
Cuspidati Rhizoma et Radix;
Emodin-8-O-β-D-glucopyranoside;
High-speed counter-current chromatography;
HPLC;
Macroporous resin;
Separation and purification
- From:
Chinese Traditional and Herbal Drugs
2016;47(12):2118-2122
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an effective and rapid method for the preparation of emodin-8-O-β-D-glucopyranoside (EG) reference substance from Cuspidati Rhizoma et Radix (PCRR). Methods: The target fraction was isolated by macroporous resin column chromatography and ODS column chromatography, and then the target compound was purified by high-speed counter-current chromatography (HSCCC). The structure was identified by ESI-MS and NMR spectral techniques, and its purity was determined by HPLC analysis using the main component self-comparison with no correction factor method. Results: A solvent system that consisted of dichloromethane-ethyl acetate-methanol-water (8:1:6:5) was applied to the separation based on assessment using HPLC partition coefficient method. The upper phase was used as the stationary phase, while the lower phase was used as the mobile phase. The reference substance of EG was prepared, and its purity was up to 98% in line with the standard of quantitative analysis. Conclusion: The method is suitable for the preparation of EG from PCRR, which provides a basis for the quality control of natural product and their preparations.
