Analysis on fingerprint for root barks and stem barks of Toddalia asiatica
10.7501/j.issn.0253-2670.2016.14.025
- Author:
Mao-Chen WEI
1
Author Information
1. Guiyang Xintian Pharmaceutical Co., Ltd.
- Publication Type:Journal Article
- Keywords:
Fingerprints;
HPLC;
Root barks;
Similarity;
Stem barks;
Toddalia asiatica
- From:
Chinese Traditional and Herbal Drugs
2016;47(14):2540-2544
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To promote the comprehensive utilization in the root barks and stem barks of Toddalia asiatica and to establish an HPLC method for the determination of the fingerprints for the root barks and stem barks of T. asiatica. Meanwhile, the similarity was compared by similarity evaluation software based on the fingerprints. Methods: HPLC analysis was performed on C18 (250 mm × 4.6 mm, 5 μm) with acetonitrile and 0.1% aqueous solution of phosphoric acid as mobile phase in gradient mode at the wavelength of 269 nm, and the flow rate was 1 mL/min. The similarity was calculated by comparison on the fingerprints derived from 10 bathes of T. asiatica root barks and 10 bathes of T. asiatica stem barks, all the samples were collected from the different areas in different seasons. Results: The mutualmode of HPLC fingerprints was set up and the similar degrees to the crude drugs of different sources were compared. The content ratio of active ingredients is different from samples in different areas and seasons. Conclusion: The fingerprint similarity is greater than 0.9 between T. asiatica root barks and T. asiatica stem barks, so the compositions are highly similar.
